Skip to content

Identifiler Plus

Definition

Applied Biosystems 16-plex STR kit covering the original 13 CODIS loci plus D2S1338, D19S433, and Amelogenin. Widely used through the 2010s; insufficient for CODIS 20 upload without supplementation.

Manufacturer
Applied Biosystems (Thermo Fisher)
Loci covered
16-plex: original 13 CODIS loci plus D2S1338, D19S433, Amelogenin
Era of use
Widely used through the 2010s
CODIS 20 status
Insufficient alone; needs supplemental kit for upload
Kit type
Multiplex STR amplification kit

Common questions

Why can't Identifiler Plus results be uploaded directly to the expanded CODIS core loci?+

CODIS expanded from 13 to 20 core loci in 2017. Identifiler Plus predates that expansion and does not amplify all of the newer core loci, so a lab must run a supplemental kit covering the missing loci before the full profile qualifies for upload.

Is a profile generated with Identifiler Plus still usable in casework today?+

Yes, for comparison purposes and for the loci it does cover. Many legacy profiles and reference databases were built on it, so it remains relevant for matching against older records even though newer kits are now standard for fresh CODIS uploads.

Related terms

Allelic Drop-Out
Failure of one allele of a heterozygous locus to amplify detectably, a stochastic effect of low-template amplification where sampling of limited template...
ESSplex SE QS
Qiagen kit covering the European Standard Set of 17 loci plus DYS391 and Amelogenin; optimised for EU Prüm database upload.
GlobalFiler
Applied Biosystems 25-plex STR kit covering 24 STR loci plus Amelogenin, compatible with both CODIS 20 and ESS17 requirements.
Haematin
Degradation product of haemoglobin that inhibits Taq polymerase by chelating Mg2+ ions and binding to the polymerase active site; most common inhibitor...
Humic Acid
Organic polymer from soil degradation that inhibits PCR by intercalating into double-stranded DNA and disrupting primer annealing.
Investigator 24Plex QS
Qiagen 26-plex STR kit with Quality Sensor (IPC) and covering 24 STR loci plus DYS391 and Amelogenin. GO! variant uses increased cycles...
IPC (Internal PCR Control)
A non-forensic DNA sequence spiked into the PCR reaction at fixed concentration; a shift in IPC Ct value relative to the expected...
Low-Copy-Number (LCN) PCR
PCR protocol using increased cycle numbers (typically 34 cycles) developed at the UK FSS to generate profiles from template inputs below ~100...
PowerPlex Fusion 6C
Promega 23-plex STR kit covering all 20 CODIS core loci, all 17 ESS loci, DYS391, and Amelogenin across 6 dye channels.
Probabilistic Genotyping
Software-based interpretation of complex DNA electropherograms using continuous statistical models (e.g. STRmix, TrueAllele, ArmedXpert). Generates a likelihood ratio for complex mixtures previously...

Explained in

Your journey to becoming a forensic professional starts here.

Practice with mock tests, learn from structured notes, and get your questions answered by a global forensic community, all in one place.