GlobalFiler
Definition
Applied Biosystems 25-plex STR kit covering 24 STR loci plus Amelogenin, compatible with both CODIS 20 and ESS17 requirements.
- Manufacturer
- Applied Biosystems / Thermo Fisher
- Loci covered
- 24-plex: 21 autosomal STR loci, Amelogenin, DYS391, Y-indel
- Standards compatibility
- CODIS 20 core loci and ESS17
- Category
- Multiplex STR amplification kit
Common questions
Why does compatibility with both CODIS 20 and ESS17 matter?+
A single kit covering both the US CODIS core loci and the European Standard Set lets a laboratory generate one profile usable for searching against either database, avoiding the need to run separate kits for cross-border or dual-standard casework.
How does GlobalFiler compare to older STR kits with fewer loci?+
Older kits covering 13 to 16 loci offer less discrimination power and predate CODIS expanding to 20 core loci in 2017, so GlobalFiler and similar higher-plex kits give better resolution for mixture deconvolution and international database searching.
What does the Amelogenin marker in the kit provide?+
It determines the sex of the DNA source by amplifying differently sized X and Y chromosome fragments, giving a male or female call alongside the STR profile rather than contributing to individual identification itself.
Related terms
- Allelic Drop-Out
- Failure of one allele of a heterozygous locus to amplify detectably, a stochastic effect of low-template amplification where sampling of limited template...
- ESSplex SE QS
- Qiagen kit covering the European Standard Set of 17 loci plus DYS391 and Amelogenin; optimised for EU Prüm database upload.
- Haematin
- Degradation product of haemoglobin that inhibits Taq polymerase by chelating Mg2+ ions and binding to the polymerase active site; most common inhibitor...
- Humic Acid
- Organic polymer from soil degradation that inhibits PCR by intercalating into double-stranded DNA and disrupting primer annealing.
- Identifiler Plus
- Applied Biosystems 16-plex STR kit covering the original 13 CODIS loci plus D2S1338, D19S433, and Amelogenin. Widely used through the 2010s; insufficient...
- Investigator 24Plex QS
- Qiagen 26-plex STR kit with Quality Sensor (IPC) and covering 24 STR loci plus DYS391 and Amelogenin. GO! variant uses increased cycles...
- IPC (Internal PCR Control)
- A non-forensic DNA sequence spiked into the PCR reaction at fixed concentration; a shift in IPC Ct value relative to the expected...
- Low-Copy-Number (LCN) PCR
- PCR protocol using increased cycle numbers (typically 34 cycles) developed at the UK FSS to generate profiles from template inputs below ~100...
- PowerPlex Fusion 6C
- Promega 23-plex STR kit covering all 20 CODIS core loci, all 17 ESS loci, DYS391, and Amelogenin across 6 dye channels.
- Probabilistic Genotyping
- Software-based interpretation of complex DNA electropherograms using continuous statistical models (e.g. STRmix, TrueAllele, ArmedXpert). Generates a likelihood ratio for complex mixtures previously...