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ESSplex SE QS

Definition

Qiagen kit covering the European Standard Set of 17 loci plus DYS391 and Amelogenin; optimised for EU Prüm database upload.

Manufacturer
Qiagen
Loci covered
European Standard Set (17 loci) plus DYS391 and Amelogenin
Primary use
STR profiling for EU Prum database upload
Category
Multiplex STR amplification kit

Common questions

Why does ESSplex SE QS include DYS391 alongside the autosomal loci?+

DYS391 is a Y-chromosome marker included to help resolve male DNA in mixtures with female contributors, giving analysts extra discrimination in cases such as sexual assault where autosomal loci alone can be ambiguous.

Why is this kit tied specifically to the European Standard Set?+

The European Standard Set is the fixed panel of loci EU member states agreed to share for cross-border Prum database searching, so a kit built around it produces profiles directly comparable across national databases without extra loci conversion.

What does the QS in the kit name typically indicate for this product line?+

QS variants in Qiagen STR kits add internal quantification and quality sensor loci that flag degraded or inhibited samples during the same amplification run, rather than requiring a separate quantification step beforehand.

Related terms

Allelic Drop-Out
Failure of one allele of a heterozygous locus to amplify detectably, a stochastic effect of low-template amplification where sampling of limited template...
GlobalFiler
Applied Biosystems 25-plex STR kit covering 24 STR loci plus Amelogenin, compatible with both CODIS 20 and ESS17 requirements.
Haematin
Degradation product of haemoglobin that inhibits Taq polymerase by chelating Mg2+ ions and binding to the polymerase active site; most common inhibitor...
Humic Acid
Organic polymer from soil degradation that inhibits PCR by intercalating into double-stranded DNA and disrupting primer annealing.
Identifiler Plus
Applied Biosystems 16-plex STR kit covering the original 13 CODIS loci plus D2S1338, D19S433, and Amelogenin. Widely used through the 2010s; insufficient...
Investigator 24Plex QS
Qiagen 26-plex STR kit with Quality Sensor (IPC) and covering 24 STR loci plus DYS391 and Amelogenin. GO! variant uses increased cycles...
IPC (Internal PCR Control)
A non-forensic DNA sequence spiked into the PCR reaction at fixed concentration; a shift in IPC Ct value relative to the expected...
Low-Copy-Number (LCN) PCR
PCR protocol using increased cycle numbers (typically 34 cycles) developed at the UK FSS to generate profiles from template inputs below ~100...
PowerPlex Fusion 6C
Promega 23-plex STR kit covering all 20 CODIS core loci, all 17 ESS loci, DYS391, and Amelogenin across 6 dye channels.
Probabilistic Genotyping
Software-based interpretation of complex DNA electropherograms using continuous statistical models (e.g. STRmix, TrueAllele, ArmedXpert). Generates a likelihood ratio for complex mixtures previously...

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