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Module 110 hrs9 topics

Unit I · Foundations of Forensic Science

Forensic science definition and history; physical evidence and chain of custody; crime scene; criminal investigations (deaths, assaults, sexual offences, poisoning, vehicular accidents); courts and expert testimony; FSLs, NCRB and NICFS; fundamental rights (Articles 14–22); criminal profiling and the three lie-detection tools; quality control in forensic institutions.

Start module
  1. Forensic Science: Definition, History, Scope and EthicsUGC-NET notes on what forensic science is under Indian statute, the Galton-Bertillon-Locard lineage, its modern sub-disciplines, and analyst ethics.8 min
  2. Physical Evidence: Nature, Types, Collection and Chain of CustodyUGC-NET notes on physical evidence: classification, search methods, collection and preservation, packaging, and chain-of-custody under BNSS and BSA 2023.14 min
  3. Crime Scene: Nature, Types and PreservationUGC-NET notes on crime scenes: classification, securing and cordoning, scene preservation under BNSS 2023 Section 176(3), and the SOCO workflow.13 min
  4. Criminal Investigations: Deaths, Assaults, Poisoning and Vehicular AccidentsUGC-NET notes on investigating unnatural deaths, assaults, sexual offences, poisoning, and vehicular accidents under BNSS and BNS 2023, with FSL SOPs.16 min
  5. Courts: Jurisdiction, Admissibility and Expert TestimonyUGC-NET notes on Indian court hierarchy and jurisdiction, admissibility under BSA 2023 Sections 39, 45 and 61-63, and expert testimony.15 min
  6. Organisation of FSLs, NCRB and NICFSUGC-NET notes on the Indian forensic ecosystem: DFSS, the seven CFSLs, state SFSLs, GEQDs, NCRB, NICFS, NFSU, and reporting lines under MHA.14 min
  7. Fundamental Rights (Articles 14-22) and Forensic PracticeUGC-NET notes on Articles 14-22 and how they shape Indian forensic practice, including Selvi 2010, custodial interrogation, and sample collection.14 min
  8. Criminal Profiling, Polygraphy, Narco-Analysis and Brain MappingUGC-NET notes on offender profiling, polygraph testing, narco-analysis, brain electrical oscillation signature, and the Selvi v Karnataka 2010 consent rule.15 min
  9. Quality Control Management in Forensic InstitutionsUGC-NET notes on quality systems in Indian forensic labs: NABL ISO/IEC 17025 accreditation, proficiency testing, audit trails, and DFSS quality manuals.13 min
Module 210 hrs9 topics

Unit II · Forensic Instrumentation

Microscopy (polarizing, comparison, stereoscopic, fluorescent, electron); spectrophotometry (UV, visible, IR, Raman, AAS, AES); neutron activation analysis; X-ray techniques (XRD, XRF); mass spectroscopy; chromatography (TLC, GLC, HPLC, HPTLC); hyphenated techniques (GC-MS, LC-MS, IR-MS, ICP-MS); electrophoresis (high and low voltage, immunoelectrophoresis); immunoassays.

Start module
  1. Microscopy for Forensic Science: Polarizing, Comparison, Stereoscopic, Fluorescent and ElectronFive microscope families NTA tests: polarizing, comparison, stereoscopic, fluorescence, and electron, covering resolution, application, and Indian CFSL use.12 min
  2. Spectrophotometry for Forensic Science: UV, Visible, IR, Raman, AAS and AESBeer-Lambert law, the IR fingerprint region, Raman versus IR selection rules, and AAS/AES, applied across Indian drugs, paint, GSR, and toxic-metal casework.13 min
  3. Neutron Activation Analysis (NAA) in Forensic ScienceThermal neutron capture, HPGe gamma spectrometry, and INAA versus RNAA, with historical GSR casework and why ICP-MS displaced NAA in Indian labs.9 min
  4. X-ray Techniques in Forensic Analysis: XRD, XRF and X-ray ImagingX-ray production and Bragg's law underpin XRD for crystalline phase ID and XRF for elemental analysis, used across Indian paint, glass, soil, and GSR casework.11 min
  5. Mass Spectroscopy: Principles, Instrumentation and Forensic ApplicationsIonisation methods, mass analysers, and the nitrogen rule for spectral interpretation, applied across drugs, accelerants, explosives, and toxicology.13 min
  6. Chromatographic Techniques in Forensic Science: TLC, GLC, HPLC and HPTLCStationary versus mobile phase, Rf values, and GLC detectors, covering how Indian SFSLs use TLC, GLC, HPLC, and HPTLC across drugs, ink, dye, and explosives.14 min
  7. Hyphenated Techniques in Forensic Science: GC-MS, LC-MS, IR-MS and ICP-MSWhy separation and identification are hyphenated, the four systems NTA tests (GC-MS, LC-MS, IR-MS, ICP-MS), and how CFSL Hyderabad and Chandigarh run them.13 min
  8. Electrophoresis in Forensic Science: High Voltage, Low Voltage and ImmunoelectrophoresisElectrophoretic mobility across paper, agarose, PAGE and IEF media, immunoelectrophoresis, and capillary electrophoresis in modern Indian DNA profiling.11 min
  9. Immunoassays in Forensic Science: Principle, Types, Techniques and ApplicationsAntigen-antibody specificity, RIA, ELISA, FPIA, EMIT and lateral-flow formats, the screen-then-confirm workflow, and NDPS rapid-test kits used by Indian police.11 min
Module 310 hrs9 topics

Unit III · Forensic Serology, DNA and Wildlife Forensics

Detection and identification of blood stains; determination of species of origin; blood group systems (ABO, Rh, MNS); blood-grouping techniques from stains (Lattes, absorption-elution, absorption-inhibition, mixed agglutination); seminal and other body fluids plus RBC enzymes and serum proteins of forensic significance; disputed paternity and maternity; DNA structure, genetic markers, extraction and STR profiling; DNA phenotyping and RNA profiling; wildlife forensics under the Wildlife Protection Act 1972.

Start module
  1. Detection and Identification of Blood Stains in Forensic ScienceThe three-step search, presumptive, and confirmatory workflow for bloodstains, including Kastle-Meyer, Luminol, and Teichmann, under BNSS Section 176(3).12 min
  2. Determination of Species of Origin from Blood and Body FluidsUhlenhuth precipitin, Ouchterlony double diffusion, and modern cyt-b PCR and COI barcoding for species origin, anchored at WII Dehradun and CCMB LaCONES.11 min
  3. Blood Group Systems in Forensic Science: ABO, Rh, MNS and BeyondLandsteiner's ABO system, Rh and the D antigen, MNS Kell Duffy Kidd Lewis, secretor status, and the Bombay phenotype, and their forensic significance.12 min
  4. Techniques for Determining Blood Groups from BloodstainsLattes crust, absorption-inhibition, absorption-elution, and mixed agglutination for tiny stains, ranked by sensitivity under Indian CFSL BSA 2023 workflow.11 min
  5. Body Fluids, RBC Enzymes and Serum Proteins of Forensic SignificanceSeminal-fluid and saliva detection, polymorphic RBC enzymes, and serum proteins, tracing the transition from classical serology to the DNA era.15 min
  6. Disputed Paternity and Maternity: Forensic Testing in IndiaSection 112 IEA legitimacy presumption, key case law, classical exclusion versus modern autosomal STR PI/CPI, and CDFD Hyderabad as the DNA referral lab.12 min
  7. DNA in Forensic Science: Structure, Genetic Markers, Extraction and ProfilingAutosomal STR, Y-STR, mtDNA and SNP typing, DNA extraction methods, multiplex PCR capillary electrophoresis, and the Indian DNA Data Bank under DFSS.17 min
  8. DNA Phenotyping and RNA Profiling in Forensic ScienceHIrisPlex-S for eye and hair colour, ancestry inference, mRNA and miRNA body-fluid panels, methylation age estimation, and India's admissibility frame.12 min
  9. Wildlife Forensics in India: Wildlife Protection Act 1972, Scope and EvidenceWLPA 1972 schedules and CITES, morphological and molecular species ID, and the Indian institutional ecosystem of WII Dehradun, CCMB LaCONES, and WCCB.13 min
Module 410 hrs10 topics

Unit IV · Forensic Toxicology

Analysis of ethyl alcohol in beverages, biological fluids and breath; methanol, denaturants and illicit liquors; chemicals in trap cases; metabolism and chemical examination of insecticides and pesticides, tranquillizers and sedatives, hypnotics, stimulants, narcotics, opiates and drugs of abuse with their toxicity; plant poisons; metallic poisons; extraction, isolation and clean-up procedures and identification of common poisons from viscera, tissues and body fluids.

Start module
  1. Analysis of Ethyl Alcohol in Beverages, Blood and BreathHeadspace GC-FID and the Widmark formula for ethanol quantification, breath analyzers, and MV Act and BNS 2023 culpable-driving sampling rules.14 min
  2. Analysis of Methanol, Denaturants and Illicit LiquorsMethanol toxicity via formic acid, chromotropic acid and GC-FID detection, denaturant markers, and India's country-liquor hooch tragedy casework.13 min
  3. Analysis of Chemicals in Trap Cases (Phenolphthalein, Anthrone)Phenolphthalein-sodium-carbonate hand-wash for currency-note traps under the Prevention of Corruption Act, and the ACB/CBI/Lokayukta sampling SOP.10 min
  4. Insecticides and Pesticides: Organochlorines, Organophosphates and CarbamatesOC, OP, carbamate and pyrethroid classification, cholinesterase inhibition, GC-ECD detection, and Indian mass-poisoning cases like Kasaragod endosulfan.15 min
  5. Tranquillizers, Sedatives and Hypnotics: Forensic AnalysisBarbiturates, benzodiazepines and Z-drugs, colour and immunoassay screening, GC-MS confirmation, and NDPS/Schedule H status in Indian casework.13 min
  6. Stimulants, Narcotics and Opiates: Forensic ToxicologyAmphetamines, cocaine and opiates, colour-test screening and GC-MS confirmation, and NDPS Act 1985 commercial-quantity thresholds in Indian casework.14 min
  7. Drugs of Abuse: Screening and Confirmation ProtocolsSAMHSA cut-offs, the immunoassay-to-GC-MS confirmation workflow across urine, blood and hair matrices, and India's NDPS sampling and DFSS SOPs.13 min
  8. Plant Poisons: Abrus, Ricinus, Datura, Aconite and OleanderAbrin and ricin lectins, Datura tropane alkaloids, aconitine cardiotoxicity, and oleandrin suicides, with microscopic seed ID and chromogenic confirmation.14 min
  9. Metallic Poisons: Arsenic, Mercury, Lead and ThalliumReinsch and Marsh tests, AAS/ICP-MS quantification, hair and nail segmental analysis for chronic exposure, and India's arsenicosis and lead-paint casework.14 min
  10. Extraction, Isolation and Clean-up of Poisons from VisceraStas-Otto fractionation, steam distillation and microdiffusion, modern SPE and QuEChERS cleanup, and the Indian DFSS viscera-analysis SOP.15 min
Module 510 hrs9 topics

Unit V · Forensic Ballistics and Tool Marks

Firearms types, classification, ammunition and compositions; forensic examination of firearms, bullets, shells and cartridges; gunshot residue analysis; velocity, penetration, dispersion, ricochet, accidental discharge and range determination; examination of country-made firearms; internal, external and terminal ballistics; tool marks; restoration of erased markings on metal surfaces.

Start module
  1. Firearms: Types, Classification, Ammunition and CompositionsRifled versus smoothbore weapons, cartridge anatomy, primer chemistry, and the Arms Act 1959 and Arms Rules 2016 frame for licensed and prohibited bores.13 min
  2. Forensic Examination of Firearms, Bullets, Shells and CartridgesComparison microscopy, striation and breech-face matching, the AFTE conclusion scale, and the Indian CFSL Chandigarh ballistics workflow.14 min
  3. Gunshot Residue (GSR) Analysis: Detection MethodsModified Griess and sodium rhodizonate colour tests, SEM-EDX detection of the Pb-Ba-Sb particle, and Indian adhesive-lift collection SOPs.14 min
  4. Velocity, Penetration, Dispersion, Ricochet and Accidental DischargeMuzzle velocity, ordnance-gelatin penetration, shotgun pellet dispersion, ricochet transfer smears, and accidental-discharge investigation methods.13 min
  5. Determination of Range of Firing in Firearm CasesContact, close, intermediate and distant shot ranges, powder tattooing and stellate wounds, and the Walker and Griess test comparison with test-fired panels.14 min
  6. Examination of Country-Made Firearms (Kattas and Tamanchas)Desi katta and pipe-gun geometry, identifying improvised versus factory weapons, and the Munger and Khargone arms-trafficking corridors under the Arms Act.12 min
  7. Internal, External and Terminal Ballistics: BasicsInternal ballistics (ignition, propellant burn), external ballistics (trajectory, drag), and terminal ballistics (cavity, fragmentation, yaw and tumble).13 min
  8. Tool Marks: Meaning, Types and ExaminationImpressed, striated and combination marks, Mikrosil casting, comparison-microscope matching, and Indian tool-mark casework in housebreaking and theft.12 min
  9. Restoration of Erased Markings on Metal SurfacesChemical etching of the cold-worked strained zone using Fry's, Davis, and copper-chloride reagents to restore erased marks in Arms Act casework.13 min
Module 610 hrs7 topics

Unit VI · Fire, Arson, Explosives, IEDs and Forensic Photography

Analyses of petroleum products and other incendiary materials; fire and arson investigation, cause-origin determination, burn patterns; explosives definition, types and analyses; post-blast residue analysis; country-made bombs and IEDs and their examination; investigation in explosion and arson cases; forensic photography types and applications in criminal investigation and evidence examination.

Start module
  1. Analysis of Petroleum Products and Fire AccelerantsGasoline, kerosene and diesel as ignitable liquids, ASTM classification, headspace SPME-GC-MS, and the CFSL Hyderabad fire-lab SOP.14 min
  2. Fire and Arson Investigation: Cause, Origin and Burn PatternsThe fire tetrahedron, V-patterns and pour patterns for origin, NFPA 921 methodology, and distinguishing accidental from incendiary fires.14 min
  3. Explosives: Definition, Types and ClassificationDeflagrating versus detonating explosives, primary, secondary and tertiary categories, and the Indian Explosives Act 1884 and Explosive Substances Act 1908.14 min
  4. Forensic Analysis of Explosives and Post-Blast ResiduesColour tests, GC-ECD, IMS and Raman for post-blast residue, and the residue-swab SOP behind the NSG/NIA/CFSL post-blast workflow.15 min
  5. Country-Made Bombs and Improvised Explosive Devices (IEDs)Pipe, pressure-cooker and tiffin bombs, the IED four-component model, render-safe procedures, and Indian terror and Naxal casework.14 min
  6. Investigation of Explosion and Arson CasesScene management under BNSS Section 176, seat-of-explosion identification, blast over-pressure mapping, and the NSG/NIA Bomb Disposal Squad workflow.13 min
  7. Forensic Photography: Types and ApplicationsThe overall, mid-range and close-up photography triad, UV/IR and alternate-light imaging, and BSA Section 39 admissibility for Indian CFSL casework.14 min
Module 710 hrs8 topics

Unit VII · Trace Evidence and Cyber Forensics

Hair and fibers (nature, types, structure, examination); pollens and diatoms in forensic investigation; dust and soil examination; paint, lacquer and varnishes; glass composition, fractures and examination; cement, mortar and concrete; computer forensics with digital-evidence seizure and acquisition; mobile phone forensics.

Start module
  1. Hair and Fibers: Nature, Types, Structure and ExaminationHair morphology and fibre typing by microscopy, FTIR and pyrolysis-GC-MS, feeding the Indian SFSL trace-evidence workflow.14 min
  2. Pollens and Diatoms in Forensic InvestigationForensic palynology for geo-locating suspects and the diatom test for drowning, applied in Indian drowning cases and post-mortem geolocation.13 min
  3. Dust and Soil: Nature, Types and Forensic ExaminationSoil composition, particle-size and colour comparison, and XRD mineral phase ID, applied to Indian hit-and-run and body-disposal casework.13 min
  4. Paint, Lacquer and Varnishes: Nature, Composition and Forensic ExaminationPaint layer composition and multi-layer analysis by SEM-EDX, FTIR and micro-Raman, applied to Indian hit-and-run paint-chip transfer cases.14 min
  5. Glass: Composition, Types, Fractures and ExaminationGlass types, refractive index by GRIM3 oil immersion, and fracture analysis distinguishing radial from concentric breaks in Indian SFSL casework.14 min
  6. Cement, Mortar and Concrete: Composition and Forensic AnalysisOPC, PPC and PSC composition and XRD/SEM-EDX phase ID, applied to IED debris, building-collapse, and Indian construction-fraud investigation.12 min
  7. Computer Forensics: Cyber Crimes, Digital Evidence, Seizure and AcquisitionCyber-crime taxonomy under the IT Act 2000, live versus dead acquisition, bit-stream imaging, and the BSA 2023 Section 63 certificate requirement.15 min
  8. Mobile Phone Forensics: Acquisition and ExaminationLogical, file-system, physical, JTAG and chip-off acquisition, SQLite app-database recovery, and cell-tower CDR analysis in Indian investigations.15 min
Module 810 hrs5 topics

Unit VIII · Fingerprints, Track Marks, Biometrics and Voice

Fingerprints (history, characteristics, types, classification, preservation, development, lifting, comparison, chance prints, computerisation, AFIS); track marks (foot prints, shoe prints, tire marks, preservation, casting, comparison, skid marks, gait); biometric systems of identification and their relevance; voice analysis (vocal apparatus, voice spectrography, speaker identification, legal aspects and limitations).

Start module
  1. Fingerprints: History, Characteristics, Types and ClassificationGalton and Henry classification developed at Indian FPB Kolkata, friction-ridge biology and minutiae types, and the NCRB Central Fingerprint Bureau.14 min
  2. Fingerprint Development, Lifting and AFIS ComparisonLatent print development by powder, ninhydrin and cyanoacrylate fuming, ACE-V comparison, NAFIS/AFIS matching, and BSA Section 39 admissibility.15 min
  3. Track Marks: Foot, Shoe, Tire, Skid Marks and Gait PatternFootwear and tyre-tread comparison, skid-mark speed estimation via drag factor, and gait-pattern analysis including CCTV gait comparison.13 min
  4. Biometric Systems of Identification and Their RelevancePhysiological versus behavioural biometrics, FAR/FRR/EER performance metrics, the UIDAI Aadhaar architecture, and presentation-attack detection.13 min
  5. Voice Analysis: Spectrography, Speaker Identification and Legal AspectsVocal apparatus and voice spectrograms, aural-spectrographic versus automatic speaker recognition, and India's admissibility post Selvi v Karnataka 2010.14 min
Module 910 hrs9 topics

Unit IX · Questioned Documents

Documents (definition, types, preliminary examination); reproduction of documents through photographic and mechanical means; alterations (erasures, obliterations, additions); indentations, secret writings and charred documents; inks, papers and age of documents; examination of typescripts, printed matter, currency notes, lottery tickets and mechanical impressions; handwritings (class and individual characteristics, factors, standard samples, comparison); anonymous and disguised writings; identification of signatures, detection of forgeries; examination of credit cards and similar materials.

Start module
  1. Documents: Definition, Types and Preliminary ExaminationDocument definition under BSA 2023 Section 2(1)(d), the preliminary examination workflow, and chain-of-custody for CFSL/SFSL QD division submission.13 min
  2. Reproduction of Documents: Photographic and Mechanical MeansPhotographic and mechanical document reproduction, identifying the source machine from banding and drum defects, and admissibility under BSA 2023.13 min
  3. Examination of Alterations: Erasures, Obliterations and AdditionsMechanical and chemical erasures, obliterations and interlineations, detected by oblique and UV/IR light and ESDA, in Indian tampered-document case law.14 min
  4. Indentations, Secret Writings and Charred DocumentsIndented-writing recovery via ESDA, secret-writing detection, and charred-document recovery techniques used in Indian terror and ransom-note casework.13 min
  5. Inks, Papers and Age of DocumentsInk composition and comparison by TLC, Raman and FTIR, paper fibre and watermark analysis, and ink-age dating by the Aginsky and Brunelle methods.15 min
  6. Typescripts, Printed Matter, Currency Notes, Lottery Tickets and Mechanical ImpressionsTypewriter and printer individuality, Indian currency security features, and counterfeit and lottery-ticket examination under FICN investigation.15 min
  7. Handwriting: Class and Individual Characteristics, Factors, Standard SamplesClass versus individual handwriting characteristics, ASTM E2290 features, and standard sample collection rules under BSA Section 39.15 min
  8. Anonymous and Disguised Writings; Signature and Forgery DetectionAnonymous-letter and disguised-writing analysis, signature forgery types, Section 463-465 BNS provisions, and key Indian admissibility precedents.16 min
  9. Forensic Examination of Credit Cards and Similar MaterialsCard anatomy and EMV/magnetic-stripe forensics, skimming and cloning detection, and IT Act Section 66C/66D provisions with RBI fraud monitoring.13 min
Module 1010 hrs8 topics

Unit X · Forensic Medicine and Anthropology

Modes and manner of death, sexual offences and amendments under BNS 2023 / POCSO; post-mortem examination, post-mortem changes, estimation of time since death; injuries and wounds (types, medicolegal importance, gunshot wounds); determination of species of origin, sex, age, stature and individual identification through skeletal remains; identification through skull superimposition and facial reconstruction; human dentition, type of teeth, age estimation and bite marks; forensic entomology (insects of forensic importance, carrion succession, applications).

Start module
  1. Modes and Manner of Death: Forensic ThanatologyCause versus mechanism versus manner of death, the three classical modes and five NAME manners, and BNSS Section 194 inquest procedure.14 min
  2. Sexual Offences: Medicolegal Importance and AmendmentsRape definition under BNS 2023 Sections 63-71, the two-finger test ban, and medicolegal examination under the modified Goa Medical Protocol.16 min
  3. Post-Mortem Examination, Post-Mortem Changes and Time Since DeathBNSS Section 194 post-mortem workflow, primary and secondary decomposition changes, and time-since-death estimation methods like Henssge's nomogram.16 min
  4. Injuries and Wounds: Types, Medicolegal Importance and Gunshot WoundsMechanical and thermal injury classification, simple versus grievous hurt under BNS 2023, antemortem versus postmortem distinction, and gunshot wounds.16 min
  5. Skeletal Remains: Species, Sex, Age, Stature and Individual IdentificationDetermining species, sex, age and stature from skeletal remains, including Indian long-bone regression formulae and DNA-based individual identification.17 min
  6. Skull Superimposition and Facial ReconstructionVideo superimposition and Manchester-method facial reconstruction, Indian soft-tissue thickness data, and casework at AIIMS Delhi and CFSL Kolkata.15 min
  7. Human Dentition: Types of Teeth, Age Estimation and Bite MarksDental formula and age estimation methods, bite-mark analysis under ABFO guidelines, and Indian admissibility post Krishan Kumar Malik 2011.15 min
  8. Forensic Entomology: Insects of Forensic Importance and ApplicationsCarrion-succession blowflies, accumulated degree days for post-mortem interval, and Indian species anchored at ICMR-VCRC and NIE Bengaluru.16 min

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