RFLP (Restriction Fragment Length Polymorphism)
Definition
The first DNA profiling method used in forensic casework, developed by Alec Jeffreys in 1984. Restriction enzymes cut DNA at specific sequences, producing fragments whose lengths vary between individuals. The pattern of fragment lengths, visualised by Southern blotting, constitutes a DNA profile. Requires relatively large, intact DNA samples.
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Explained in these topics
- History and Development of Forensic BiologyThe first DNA profiling method used in forensic casework, developed by Alec Jeffreys in 1984. Restriction enzymes cut DNA at specific sequences, producing frag...
- Restriction Enzymes, Vectors, Ligation and Molecular CloningVariation in the length of DNA fragments produced by digestion of genomic DNA with a restriction enzyme, due to differences in the spacing between restriction...