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Blue-white screening (alpha-complementation)

A colony selection method for pUC-family vectors in which intact lacZ-alpha produces functional beta-galactosidase that cleaves X-gal to a blue indigo product. Insertion of foreign DNA into the MCS disrupts the reading frame, giving white colonies.

Explained in

Restriction Enzymes, Vectors, Ligation and Molecular Cloning

Related terms

Bacterial artificial chromosome (BAC)Multiple cloning site (MCS)Plasmid RFLP (restriction fragment length polymorphism)Sticky end (cohesive end)Type II restriction endonuclease

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