Real-time PCR quantification
Definition
A method that measures the concentration of human-specific amplifiable DNA in a forensic extract by monitoring fluorescent signal accumulation during PCR in real time. Modern kits simultaneously report total human DNA, male DNA from the Y chromosome, and a degradation index from the ratio of short-to-long target amplification.
Related terms
- Chelex extraction
- A rapid extraction method that uses the chelating resin Chelex 100 to bind divalent metal ions and prevent nuclease activity. Cells are...
- Differential extraction
- A two-step DNA extraction procedure used when a stain contains both sperm cells and non-sperm epithelial cells. The first step lyses epithelial...
- Organic extraction
- A DNA isolation method that uses phenol and chloroform to partition proteins into the organic phase, leaving nucleic acids in the aqueous...
- PCR inhibitor
- A co-extracted substance that interferes with Taq polymerase activity or primer annealing. Common inhibitors in forensic samples include haemoglobin (from blood on...
- Solid-phase extraction
- A family of methods in which DNA binds selectively to a silica membrane or magnetic bead under high-salt, low-pH conditions, is washed...
Explained in
- DNA Extraction and Quantification from Biological EvidenceA method that measures the concentration of human-specific amplifiable DNA in a forensic extract by monitoring fluorescent signal accumulation during PCR in re...