Chelex extraction
Definition
A rapid extraction method that uses the chelating resin Chelex 100 to bind divalent metal ions and prevent nuclease activity. Cells are boiled directly in the Chelex suspension. The method is simple and inexpensive but yields single-stranded DNA and is not suitable for restriction fragment length polymorphism analysis.
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Explained in
- DNA Extraction and Quantification from Biological EvidenceA rapid extraction method that uses the chelating resin Chelex 100 to bind divalent metal ions and prevent nuclease activity. Cells are boiled directly in the...