Organic extraction
Definition
A DNA isolation method that uses phenol and chloroform to partition proteins into the organic phase, leaving nucleic acids in the aqueous phase. The gold standard for yield and purity but requires hazardous chemical handling and is largely replaced by solid-phase methods in high-throughput laboratories.
Related terms
- Chelex extraction
- A rapid extraction method that uses the chelating resin Chelex 100 to bind divalent metal ions and prevent nuclease activity. Cells are...
- Differential extraction
- A two-step DNA extraction procedure used when a stain contains both sperm cells and non-sperm epithelial cells. The first step lyses epithelial...
- PCR inhibitor
- A co-extracted substance that interferes with Taq polymerase activity or primer annealing. Common inhibitors in forensic samples include haemoglobin (from blood on...
- Real-time PCR quantification
- A method that measures the concentration of human-specific amplifiable DNA in a forensic extract by monitoring fluorescent signal accumulation during PCR in...
- Solid-phase extraction
- A family of methods in which DNA binds selectively to a silica membrane or magnetic bead under high-salt, low-pH conditions, is washed...
Explained in
- DNA Extraction and Quantification from Biological EvidenceA DNA isolation method that uses phenol and chloroform to partition proteins into the organic phase, leaving nucleic acids in the aqueous phase. The gold stand...