QuantiBlot
Definition
Human-specific slot-blot quantitation kit (Applied Biosystems) using a probe directed at the D17Z1 alpha-satellite repeat on chromosome 17.
- Manufacturer
- Applied Biosystems
- Method
- Slot-blot quantitation
- Target
- D17Z1 alpha-satellite repeat, chromosome 17
- Specificity
- Human-specific
Common questions
Why did labs move away from slot-blot kits like QuantiBlot toward qPCR-based quantification?+
qPCR methods such as Quantifiler provide a numeric DNA concentration with a wider dynamic range and better precision in a fraction of the time, and can simultaneously assess degradation and inhibition, advantages a slot-blot hybridisation assay does not offer.
Does QuantiBlot distinguish male from female DNA in a sample?+
No. It targets a chromosome-17 repeat present in both sexes, so it reports total human DNA quantity only; a separate assay is needed to determine the male DNA fraction in a mixed sample.
Related terms
- A260/A280 Ratio
- Absorbance ratio at 260 nm and 280 nm used to assess nucleic acid purity. Values of 1.7-1.9 indicate pure DNA; lower values...
- Cq (Cycle Threshold)
- The PCR cycle at which fluorescence crosses a defined threshold; inversely proportional to starting template concentration and used to calculate DNA quantity...
- Degradation Index (DI)
- The ratio of large-amplicon to small-amplicon Cq values in Quantifiler Trio or equivalent kits; a DI above ~3 indicates significant fragmentation of...
- InnoQuant HY
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- PicoGreen
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- Plexor HY
- Promega qPCR quantitation kit using isoguanosine/isocytidine quenching chemistry, providing autosomal and Y-chromosome quantitation with degradation index.
- Quantifiler Trio
- Applied Biosystems qPCR kit reporting autosomal total DNA (small + large amplicon targets) and a Y-chromosome target, with a computed Degradation Index...