Plexor HY
Definition
Promega qPCR quantitation kit using isoguanosine/isocytidine quenching chemistry, providing autosomal and Y-chromosome quantitation with degradation index.
- Manufacturer
- Promega
- Method
- Quantitative PCR (qPCR)
- Quenching chemistry
- Isoguanosine / isocytidine base pair
- Targets quantified
- Autosomal DNA and Y-chromosome DNA
- Extra output
- Degradation index
Common questions
What does the degradation index reported by Plexor HY tell an analyst?+
It compares amplification of a larger versus a smaller target to estimate how fragmented the DNA sample is, flagging degraded evidence before the analyst commits to a full STR workflow that a badly degraded sample would not support.
Why quantify Y-chromosome DNA alongside total autosomal DNA?+
The separate male target reveals whether a mixed stain contains a male contributor and roughly how much male DNA is present relative to the total, which guides decisions in sexual-assault casework where female DNA can dominate the mixture.
Related terms
- A260/A280 Ratio
- Absorbance ratio at 260 nm and 280 nm used to assess nucleic acid purity. Values of 1.7-1.9 indicate pure DNA; lower values...
- Cq (Cycle Threshold)
- The PCR cycle at which fluorescence crosses a defined threshold; inversely proportional to starting template concentration and used to calculate DNA quantity...
- Degradation Index (DI)
- The ratio of large-amplicon to small-amplicon Cq values in Quantifiler Trio or equivalent kits; a DI above ~3 indicates significant fragmentation of...
- InnoQuant HY
- Human-specific qPCR quantitation kit with internal PCR control, providing autosomal and Y quantitation plus degradation index; widely used in Japanese and East...
- PicoGreen
- SYBR Green I-related fluorescent dye that intercalates specifically into double-stranded DNA, used for sensitive fluorometric quantitation in plate-reader or Qubit formats.
- QuantiBlot
- Human-specific slot-blot quantitation kit (Applied Biosystems) using a probe directed at the D17Z1 alpha-satellite repeat on chromosome 17.
- Quantifiler Trio
- Applied Biosystems qPCR kit reporting autosomal total DNA (small + large amplicon targets) and a Y-chromosome target, with a computed Degradation Index...