InnoQuant HY
Definition
Human-specific qPCR quantitation kit with internal PCR control, providing autosomal and Y quantitation plus degradation index; widely used in Japanese and East Asian forensic laboratories.
- Field
- Forensic DNA quantification
- Method
- Human-specific qPCR
- Outputs
- Autosomal quantity, Y quantity, degradation index
- Region of use
- Widely used in Japan and East Asia
Common questions
Why does a quantification kit report a degradation index alongside DNA quantity?+
The degradation index compares amplification of a long versus a short target region, and a large difference signals fragmented DNA. This tells the analyst not just how much DNA is present but whether downstream STR typing is likely to succeed.
Why is separate Y-chromosome quantitation useful in casework?+
Reporting male DNA quantity alongside total autosomal DNA helps the analyst judge whether a mixed male-female sample, such as from a sexual assault case, contains enough male template to proceed with differential extraction or Y-STR typing.
Related terms
- A260/A280 Ratio
- Absorbance ratio at 260 nm and 280 nm used to assess nucleic acid purity. Values of 1.7-1.9 indicate pure DNA; lower values...
- Cq (Cycle Threshold)
- The PCR cycle at which fluorescence crosses a defined threshold; inversely proportional to starting template concentration and used to calculate DNA quantity...
- Degradation Index (DI)
- The ratio of large-amplicon to small-amplicon Cq values in Quantifiler Trio or equivalent kits; a DI above ~3 indicates significant fragmentation of...
- PicoGreen
- SYBR Green I-related fluorescent dye that intercalates specifically into double-stranded DNA, used for sensitive fluorometric quantitation in plate-reader or Qubit formats.
- Plexor HY
- Promega qPCR quantitation kit using isoguanosine/isocytidine quenching chemistry, providing autosomal and Y-chromosome quantitation with degradation index.
- QuantiBlot
- Human-specific slot-blot quantitation kit (Applied Biosystems) using a probe directed at the D17Z1 alpha-satellite repeat on chromosome 17.
- Quantifiler Trio
- Applied Biosystems qPCR kit reporting autosomal total DNA (small + large amplicon targets) and a Y-chromosome target, with a computed Degradation Index...