Indicator Cells
Definition
Commercially prepared or freshly washed red cells of known ABO group used to detect antibody activity in an eluate or residual antiserum. A-positive cells detect anti-A activity; B-positive cells detect anti-B activity.
Related terms
- Absorption
- The purification technique in which a crude antiserum is mixed with antigen preparations from cross-reacting species. Cross-reactive antibodies bind those antigens and...
- Elution
- The release of antibody from antigen, typically by heating to 56-70 degrees Celsius. In the absorption-elution method, this step liberates the antibody...
- Inhibition Index
- A quantitative measure of PCR inhibitor burden in a DNA extract, derived from the Ct shift of an internal positive control (IPC)...
- Mixed Agglutination
- A microscopy-based technique in which indicator cells form rosette aggregates around antigen-bearing stain material via antibody bridges. Positive results are read as...
- Titre
- The concentration of antibody in a solution, expressed as the reciprocal of the highest dilution at which agglutination is still visible. A...
Explained in
- ABO Typing on Stains: Absorption-Elution and InhibitionCommercially prepared or freshly washed red cells of known ABO group used to detect antibody activity in an eluate or residual antiserum. A-positive cells dete...