Titre
Definition
The concentration of antibody in a solution, expressed as the reciprocal of the highest dilution at which agglutination is still visible. A titre of 64 means agglutination was seen at 1:64 dilution. Titre reduction in inhibition tests indicates that antigen was present in the stain extract.
Related terms
- Absorption
- The purification technique in which a crude antiserum is mixed with antigen preparations from cross-reacting species. Cross-reactive antibodies bind those antigens and...
- Adjuvant
- A substance co-administered with an antigen to amplify the immune response. Freund's Complete Adjuvant (FCA), containing killed mycobacteria in mineral oil, is...
- Antiserum
- Serum collected from an immunised animal containing antibodies directed against a specific antigen or group of antigens. In species identification, antiserum raised...
- Elution
- The release of antibody from antigen, typically by heating to 56-70 degrees Celsius. In the absorption-elution method, this step liberates the antibody...
- Indicator Cells
- Commercially prepared or freshly washed red cells of known ABO group used to detect antibody activity in an eluate or residual antiserum....
- Inhibition Index
- A quantitative measure of PCR inhibitor burden in a DNA extract, derived from the Ct shift of an internal positive control (IPC)...
- Mixed Agglutination
- A microscopy-based technique in which indicator cells form rosette aggregates around antigen-bearing stain material via antibody bridges. Positive results are read as...
- Monospecific Antiserum
- An antiserum that reacts exclusively with proteins from the target species after absorption against all cross-reacting species has been performed and verified....
- Phylogenetic Cross-Reactivity
- The reaction of an antiserum with proteins from non-target species whose proteins are structurally similar due to shared evolutionary ancestry. Cross-reactivity intensity...
Explained in these topics
- ABO Typing on Stains: Absorption-Elution and InhibitionThe concentration of antibody in a solution, expressed as the reciprocal of the highest dilution at which agglutination is still visible. A titre of 64 means a...
- Antisera Production, Standardisation, and Cross-ReactivityThe highest dilution of an antiserum that still produces a detectable reaction with the target antigen under defined test conditions. Titre is the primary meas...