Elution
Definition
The release of antibody from antigen, typically by heating to 56-70 degrees Celsius. In the absorption-elution method, this step liberates the antibody captured on the stain so it can be identified by testing against known cells.
Related terms
- Absorption
- In serology, the process of adding antigen to an antiserum so that specific antibodies bind to the antigen and are removed from...
- Indicator cells
- Commercially prepared or freshly washed red cells of known ABO group used to detect antibody activity in an eluate or residual antiserum....
- Inhibition index
- A quantitative measure of PCR inhibitor burden in a DNA extract, derived from the Ct shift of an internal positive control (IPC)...
- Mixed agglutination
- A microscopy-based technique in which indicator cells form rosette aggregates around antigen-bearing stain material via antibody bridges. Positive results are read as...
- Titre
- The concentration of antibody in a solution, expressed as the reciprocal of the highest dilution at which agglutination is still visible. A...
Explained in
- ABO Typing on Stains: Absorption-Elution and InhibitionThe release of antibody from antigen, typically by heating to 56-70 degrees Celsius. In the absorption-elution method, this step liberates the antibody capture...