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Species Complex

Definition

A group of closely related species that are nearly identical in morphology but behaviourally or ecologically distinct. Molecular methods are often the only way to separate them, and incorrect identification within a complex can shift a PMI estimate by hours.

Field
Forensic entomology, taxonomy
Defining trait
Near-identical morphology, distinct behaviour or ecology
Resolution method
Molecular identification (DNA sequencing)
Casework impact
Misidentification can shift a PMI estimate by hours

Common questions

Why can two species in a complex give different PMI estimates?+

Species within a complex can differ in development rate at a given temperature even though they look alike, so assigning the wrong species to a larval sample carries the wrong growth-rate data into the postmortem interval calculation.

Why not just rely on morphology under a microscope?+

Morphological features overlap so closely between species in a complex that visual keys cannot separate them reliably, which is why DNA barcoding is used as the standard resolution method in casework.

Related terms

BOLD
Barcode of Life Data System, an international curated database linking COI sequences to verified voucher specimens with collection locality and taxonomic annotation....
COI (Cytochrome C Oxidase I)
A mitochondrial gene encoding subunit I of the respiratory enzyme cytochrome c oxidase. Its intermediate evolutionary rate makes it the primary barcode...
Cytochrome B (Cyt B)
A mitochondrial protein-coding gene widely used in forensic species identification before COI barcoding was standardised. Still used because its reference database for...
DNA Barcoding
Species identification using a short, standardised region of the mitochondrial genome. For animals, the primary barcode marker is a 648-base-pair segment of...
Morphological Identification
Species determination based on external or internal physical features. Reliable and cheap for adults and many late-instar larvae, but fails on early...

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