RSID-Saliva
Definition
Rapid Stain Identification of Saliva. An immunochromatographic lateral-flow assay using monoclonal antibodies specific to human salivary amylase. More specific than Phadebas, with fewer cross-reactions with non-salivary amylase sources.
Related terms
- ABAcard P30
- A lateral-flow immunochromatographic strip manufactured by Abacus Diagnostics and validated by the Scientific Working Group for DNA Analysis Methods (SWGDAM) for forensic...
- Alpha-Amylase (Salivary)
- An enzyme encoded by the AMY1 gene and secreted by the parotid, submandibular, and sublingual glands. It cleaves starch at alpha-1,4-glycosidic bonds...
- Azoospermia
- The absence of spermatozoa from ejaculated semen, resulting from obstructive causes (vasectomy, ductal blockage) or non-obstructive causes (testicular failure). Azoospermic semen contains...
- Double-Swab Technique
- A collection method for dried biological stains in which a moistened swab rehydrates and lifts the stain from the surface, followed immediately...
- Isoforms of Amylase
- The human genome encodes multiple AMY loci. AMY1 products are expressed in saliva; AMY2 products are expressed in the pancreas. The two...
- Lateral-Flow Immunochromatographic Assay
- A rapid, single-use immunoassay format in which sample extract migrates along a nitrocellulose membrane past a detection zone loaded with antigen-specific antibody...
- Phadebas Test
- A colorimetric presumptive test for alpha-amylase activity. Phadebas tablets contain a blue-dyed insoluble starch; amylase releases soluble blue dye that is measured...
- Presumptive Vs. Confirmatory Testing
- Presumptive tests (Phadebas) give rapid screening results but have cross-reactants; they justify further analysis. Confirmatory or highly specific tests (RSID-Saliva, mRNA analysis)...
- PSA / P30
- Prostate-specific antigen, a kallikrein-family serine protease secreted by prostatic epithelium. Present in seminal plasma at 0.5 to 2 mg/mL. The standard confirmatory...
- Salivary Alpha-Amylase
- An enzyme (EC 3.2.1.1) secreted by the parotid, submandibular, and sublingual glands. Digests starch and is present in saliva at concentrations of...
Explained in these topics
- Immunological Identification of Semen and SalivaRapid Stain Identification of Saliva: a lateral-flow strip test from Independent Forensics targeting salivary alpha-amylase. Validated to detect saliva at 1:10...
- Saliva Identification: Amylase and RSID-SalivaRapid Stain Identification of Saliva. An immunochromatographic lateral-flow assay using monoclonal antibodies specific to human salivary amylase. More specific...