ABAcard P30
Definition
A lateral-flow immunochromatographic strip manufactured by Abacus Diagnostics and validated by the Scientific Working Group for DNA Analysis Methods (SWGDAM) for forensic semen identification. Detection threshold: 4 ng/mL PSA in aqueous extract.
Related terms
- Azoospermia
- The absence of spermatozoa from ejaculated semen, resulting from obstructive causes (vasectomy, ductal blockage) or non-obstructive causes (testicular failure). Azoospermic semen contains...
- Confirmatory Test
- A test with sufficient specificity to establish the identity of a biological fluid for reporting purposes. Examples include Takayama and Teichmann crystal...
- Kastle-Meyer (KM) Test
- Phenolphthalein-based colorimetric presumptive blood test relying on haem peroxidase activity. Positive result: pink colour within 2-5 seconds of hydrogen peroxide addition.
- Lateral-Flow Immunochromatographic Assay
- A rapid, single-use immunoassay format in which sample extract migrates along a nitrocellulose membrane past a detection zone loaded with antigen-specific antibody...
- Luminol
- A chemiluminescent reagent (5-amino-2,3-dihydro-1,4-phthalazinedione) that produces blue-white light on contact with haemoglobin-derived iron. Detects blood diluted to 1:10,000,000 or more. Used as...
- mRNA Profiling
- Detection of messenger RNA transcripts specific to particular tissues or body fluids. Endometrial-specific transcripts (LEFTY2, MMP-10 mRNA, others) can distinguish menstrual blood...
- Presumptive Test
- A fast, low-cost, class-specific screen used to triage a caseload before instrumental work begins. Examples are Marquis reagent for opioids and amphetamines,...
- PSA / P30
- Prostate-specific antigen, a kallikrein-family serine protease secreted by prostatic epithelium. Present in seminal plasma at 0.5 to 2 mg/mL. The standard confirmatory...
- RSID-Saliva
- Rapid Stain Identification of Saliva. An immunochromatographic lateral-flow assay using monoclonal antibodies specific to human salivary amylase. More specific than Phadebas, with...
- RSID Strips (Independent Forensics)
- Lateral-flow immunochromatographic strips for confirmatory identification of blood (glycophorin A), semen (semenogelin I), saliva (salivary amylase), urine (urea transporter UT-A), vaginal fluid...
- Salivary Alpha-Amylase
- An enzyme (EC 3.2.1.1) secreted by the parotid, submandibular, and sublingual glands. Digests starch and is present in saliva at concentrations of...
Explained in these topics
- Body-Fluid Identification: Presumptive and Confirmatory TestsLateral-flow immunochromatographic strip detecting prostate-specific antigen (PSA) in seminal stains. Used as a confirmatory test for semen; detects PSA at con...
- Immunological Identification of Semen and SalivaA lateral-flow immunochromatographic strip manufactured by Abacus Diagnostics and validated by the Scientific Working Group for DNA Analysis Methods (SWGDAM) f...