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Quantifier and qualifier transitions

The most intense MRM transition is the quantifier and is used for the concentration calculation. The second (and sometimes third) transition is the qualifier and confirms identity. The ratio of quantifier to qualifier intensity in the sample must match the same ratio in a reference standard within plus or minus 20 to 30 percent.

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Related terms

Accurate mass and mass defectCID and HCDDeuterated internal standardMRM (multiple reaction monitoring)Tandem MS (MS/MS)

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