Ministr (reduced-size) STR
Definition
A short tandem repeat assay redesigned so that the amplicon spans a shorter distance from primer to primer, typically less than 200 base pairs. Ministr assays recover profiles from severely fragmented DNA that standard STR kits (amplicons up to 400 base pairs) fail to amplify.
Related terms
- 8-Oxoguanine
- An oxidised form of guanine produced by reactive oxygen species attacking the C8 position of the guanine ring. 8-Oxoguanine mispairs with adenine...
- Abasic site
- A position in the DNA backbone where the nitrogenous base has been lost, leaving only the deoxyribose-phosphate scaffold. Also called an AP...
- Depurination
- Hydrolytic cleavage of the N-glycosidic bond between deoxyribose and a purine base (adenine or guanine), generating an abasic (apurinic) site. Abasic sites...
- Internal positive control (IPC)
- An exogenous DNA template of known sequence and concentration added to the PCR reaction alongside the forensic sample. If the IPC fails...
- PCR inhibitor
- A co-extracted substance that interferes with Taq polymerase activity or primer annealing. Common inhibitors in forensic samples include haemoglobin (from blood on...
Explained in
- DNA Degradation: Mechanisms, Inhibitors and Environmental EffectsA short tandem repeat assay redesigned so that the amplicon spans a shorter distance from primer to primer, typically less than 200 base pairs. Ministr assays...