MiniSTR
Definition
An STR design reducing the amplified span where possible, with method-specific limits. Shortened STR designs can improve access to some fragmented targets, while mitochondrial analysis addresses a different inherited genome.
- Context
- DNA recovery
For example, a shorter amplified target can improve access to some fragmented material without guaranteeing that every required locus will produce a usable result.
Select the method for the material and question.
Neither method guarantees a complete profile from degraded evidence. Mitochondrial sharing and nuclear STR discrimination require different interpretation.
Related terms
- Abasic Site
- An abasic site is a position in a nucleic-acid backbone where the nitrogenous base is missing while part of the sugar–phosphate structure...
- Fluorescence Quenching
- Reduction of detected fluorescent signal through interactions with the detection system. Antigen–antibody recognition can be linked to different detection systems. Fluorescent responses...
- Inhibition
- Interference with an assay by substances or conditions, distinct from loss or fragmentation of the target. An internal control can help assess...
- Internal Amplification Control
- A target used to assess relevant reaction behaviour within an assay. Inhibition concerns interference with the assay rather than simply a low...