Fluorescence Quenching
Definition
Reduction of detected fluorescent signal through interactions with the detection system. Antigen–antibody recognition can be linked to different detection systems. Fluorescent responses can also decrease through interactions described as quenching.
- Context
- Assay mechanisms
For example, lower detected fluorescence can reflect interactions affecting emission rather than a smaller amount of the target alone.
A signal change is interpreted within the validated assay. It does not by itself establish a material’s identity, concentration or source.
Related terms
- Abasic Site
- An abasic site is a position in a nucleic-acid backbone where the nitrogenous base is missing while part of the sugar–phosphate structure...
- Inhibition
- Interference with an assay by substances or conditions, distinct from loss or fragmentation of the target. An internal control can help assess...
- Internal Amplification Control
- A target used to assess relevant reaction behaviour within an assay. Inhibition concerns interference with the assay rather than simply a low...
- MiniSTR
- An STR design reducing the amplified span where possible, with method-specific limits. Shortened STR designs can improve access to some fragmented targets,...