Internal Amplification Control
Definition
A target used to assess relevant reaction behaviour within an assay. Inhibition concerns interference with the assay rather than simply a low amount of target. A control result does not characterise every possible failure or contamination event.
- Context
- PCR performance
For example, an unexpected control response can prompt investigation of reaction interference without identifying its exact chemical cause by itself.
Interpret it using the validated test and specimen conditions.
Related terms
- Abasic Site
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- Fluorescence Quenching
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- Inhibition
- Interference with an assay by substances or conditions, distinct from loss or fragmentation of the target. An internal control can help assess...
- MiniSTR
- An STR design reducing the amplified span where possible, with method-specific limits. Shortened STR designs can improve access to some fragmented targets,...