EpiTYPER

Related terms

Bisulphite Conversion

A chemical treatment that deaminates unmethylated cytosines to uracil, converting them to thymine upon PCR amplification; methylated cytosines are protected. Allows methylation...

Cell-Free DNA (cfDNA)

Short DNA fragments (~166 bp) circulating extracellularly in body fluids, shed from apoptotic and necrotic cells. In plasma, predominantly hematopoietic; in pregnancy,...

CpG Methylation

Addition of a methyl group to cytosine in a CpG dinucleotide. Methylation at age-associated CpGs increases or decreases with chronological age in...

ELOVL2 Clock

A forensic age-estimation system based on bisulphite pyrosequencing of 5 CpG sites in the ELOVL2 gene promoter; validated in multiple independent European...

EPIC Array (Illumina)

The Infinium MethylationEPIC v2.0 microarray measuring methylation beta values at approximately 935,000 CpG sites genome-wide; the current standard instrument for multi-CpG age-clock...

HIrisPlex-S

A 41-SNP externally visible characteristics (EVC) panel for simultaneous prediction of eye, hair, and skin colour, developed at Erasmus MC Rotterdam by...

Horvath 353-CpG Clock

A pan-tissue epigenetic age clock built on the methylation values of 353 CpG sites from the Illumina 450K array; developed by Steve...

MiRNA (microRNA)

A small non-coding RNA (21-23 nt) that regulates gene expression post-transcriptionally. Different tissues and body fluids express different miRNA sets; forensic miRNA...

Explained in

• Emerging Markers: DNA Methylation, miRNA and HIrisPlex-S PhenotypingAgena Bioscience (formerly Sequenom) reagent system for quantitative methylation analysis via bisulphite PCR and MALDI-TOF mass spectrometry; used for multi-Cp...