Yfiler Plus
Definition
Commercial Y-STR multiplex kit (ThermoFisher Scientific) amplifying 27 loci in a single PCR reaction. Current standard for Y-STR casework in many US, European and Australian laboratories.
- Manufacturer
- Thermo Fisher Scientific
- Loci amplified
- 27
- Reaction
- Single multiplex PCR
- Status
- Current standard Y-STR kit in many laboratories
- Regions used
- US, European and Australian laboratories
Common questions
What advantage does a 27-locus kit like Yfiler Plus offer over older, smaller Y-STR kits?+
More loci increase the discriminatory power of the resulting haplotype and improve resolution of closely related paternal lineages, while amplifying all of them in a single reaction keeps the workflow no more complex than earlier, smaller kits.
Is a Yfiler Plus haplotype comparable to results from an older Y-STR kit?+
Only at the loci the two kits have in common; Yfiler Plus includes additional loci beyond earlier kits, so a full comparison requires both profiles were typed with a kit covering the same locus set, or that the comparison is restricted to the loci they share.
Related terms
- Argus X-12 QS
- Investigator Argus X-12 QS (Qiagen), the most widely validated commercial X-STR multiplex kit. Amplifies 12 loci in four linkage groups; used with...
- PowerPlex Y23
- Commercial Y-STR multiplex kit (Promega Corporation) amplifying 23 loci, overlapping substantially with Yfiler Plus. The 20-locus YHRD core set is covered by...
- X-STR Linkage Group
- A cluster of X-STR loci that are physically linked on the X chromosome and transmitted as a haplotype block in females. Loci...
- Y-STR Haplotype
- The combination of allele values across all Y-chromosome STR loci typed in a single analysis. Reported as a string of allele values...
- YHRD
- Y-Chromosome STR Haplotype Reference Database, maintained at the Charité Berlin and freely accessible at yhrd.org. Contains over 340,000 haplotypes from 124 countries....