Tissue-specific expression
Definition
The pattern by which certain genes are transcribed at high levels in one tissue and at negligible levels in others, forming the molecular basis for mRNA-based fluid identification.
Related terms
- mRNA (messenger RNA)
- Transcripts encoding proteins, synthesised in the nucleus from DNA templates and translated by ribosomes. mRNA molecules vary greatly in length (hundreds to...
- NanoString nCounter
- A hybridisation and digital counting platform that tallies individual mRNA molecules using fluorescent barcodes, avoiding amplification bias and enabling simultaneous profiling of...
- OSAC validation
- The framework for forensic assay validation published by the US Organisation of Scientific Area Committees, requiring demonstrations of sensitivity, specificity, mixture performance,...
- RNase
- Ribonuclease enzymes that cleave RNA phosphodiester bonds. Present in blood plasma (RNase A family) and released from cells on death. The activity...
- RT-PCR (reverse transcription PCR)
- A two-stage process: RNA is first reverse-transcribed into complementary DNA (cDNA), then the cDNA is amplified by PCR. Quantitative RT-PCR (RT-qPCR) measures...
Explained in
- mRNA Profiling for Body Fluid IdentificationThe pattern by which certain genes are transcribed at high levels in one tissue and at negligible levels in others, forming the molecular basis for mRNA-based...