RT-PCR (reverse transcription PCR)
Definition
A two-stage process: RNA is first reverse-transcribed into complementary DNA (cDNA), then the cDNA is amplified by PCR. Quantitative RT-PCR (RT-qPCR) measures transcript abundance in real time using fluorescent reporters.
Related terms
- mRNA (messenger RNA)
- Transcripts encoding proteins, synthesised in the nucleus from DNA templates and translated by ribosomes. mRNA molecules vary greatly in length (hundreds to...
- NanoString nCounter
- A hybridisation and digital counting platform that tallies individual mRNA molecules using fluorescent barcodes, avoiding amplification bias and enabling simultaneous profiling of...
- OSAC validation
- The framework for forensic assay validation published by the US Organisation of Scientific Area Committees, requiring demonstrations of sensitivity, specificity, mixture performance,...
- RNase
- Ribonuclease enzymes that cleave RNA phosphodiester bonds. Present in blood plasma (RNase A family) and released from cells on death. The activity...
- Tissue-specific expression
- The pattern by which certain genes are transcribed at high levels in one tissue and at negligible levels in others, forming the...
Explained in
- mRNA Profiling for Body Fluid IdentificationA two-stage process: RNA is first reverse-transcribed into complementary DNA (cDNA), then the cDNA is amplified by PCR. Quantitative RT-PCR (RT-qPCR) measures...