Standard Curve
Definition
A series of known antigen concentrations run on the same ELISA plate, used to convert the OD reading for an unknown sample into a concentration value in nanograms per millilitre.
Related terms
- Cross-Reactivity Matrix
- A table documenting the assay's optical density response to blood from multiple non-target species, expressed as a percentage of the human blood...
- Horseradish Peroxidase (HRP)
- The most common enzyme label in immunoassays. When the substrate TMB (3,3'-5,5'-tetramethylbenzidine) is added, HRP converts it to a blue product that...
- Limit of Detection (LOD)
- The lowest concentration of analyte that produces a signal reliably distinguishable from the instrument noise, conventionally three times the standard deviation of...
- Optical Density (OD)
- The absorbance reading from a microplate reader that measures how much light is absorbed by the coloured product. Higher OD means more...
- Sandwich ELISA
- An ELISA format in which a capture antibody immobilised on the plate binds one epitope of the target antigen, and a separate...
Explained in
- Anti-Human Haemoglobin ELISA and Modern ImmunoassaysA series of known antigen concentrations run on the same ELISA plate, used to convert the OD reading for an unknown sample into a concentration value in nanogr...