Horseradish peroxidase (HRP)
Definition
The most common enzyme label in immunoassays. When the substrate TMB (3,3'-5,5'-tetramethylbenzidine) is added, HRP converts it to a blue product that turns yellow on acidification and is read at 450 nm.
Related terms
- Cross-reactivity matrix
- A table documenting the assay's optical density response to blood from multiple non-target species, expressed as a percentage of the human blood...
- Limit of detection (LOD)
- The lowest concentration of analyte that produces a signal reliably distinguishable from the instrument noise, conventionally three times the standard deviation of...
- Optical density (OD)
- The absorbance reading from a microplate reader that measures how much light is absorbed by the coloured product. Higher OD means more...
- Sandwich ELISA
- A two-antibody ELISA format in which a capture antibody immobilised on a microplate well binds the target antigen from the sample, and...
- Standard curve
- A series of known antigen concentrations run on the same ELISA plate, used to convert the OD reading for an unknown sample...
Explained in
- Anti-Human Haemoglobin ELISA and Modern ImmunoassaysThe most common enzyme label in immunoassays. When the substrate TMB (3,3'-5,5'-tetramethylbenzidine) is added, HRP converts it to a blue product that turns ye...