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Spermatozoon (pl. spermatozoa)

Definition

The mature male gamete: an oval head (4-5 microns) containing condensed nuclear DNA and an acrosomal cap, a short midpiece containing mitochondria for motility, and a long flagellar tail (about 45-50 microns). Its presence in a stain is definitive evidence of semen.

Related terms

Acrosome
A cap-like organelle covering the anterior half of the sperm head. It contains hydrolytic enzymes used in oocyte penetration. Forensically it appears...
Azoospermia
The absence of spermatozoa in ejaculate, whether due to obstructive causes (post-vasectomy) or non-obstructive causes (spermatogenic failure). Affects roughly 1% of men...
Christmas tree staining
A two-step differential stain using Nuclear Fast Red (Kernechtrot) to stain the sperm head red/pink and Picroindigocarmine to stain the midpiece and...
Ghost head (decapitated spermatozoon)
An isolated sperm head separated from its tail, commonly found in aged or dried stains. The head retains its oval morphology and...
Sperm reporting conventions
Standardised ways to record microscopy results: some labs report a count per high-power field (HPF), others report as present/absent, and some use...

Explained in

  • Spermatozoa Identification by MicroscopyThe mature male gamete: an oval head (4-5 microns) containing condensed nuclear DNA and an acrosomal cap, a short midpiece containing mitochondria for motility...

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