Short Tandem Repeat (STR) Profiling
Definition
The amplification and typing of microsatellite loci where short DNA sequences are repeated a variable number of times. When applied to canine or feline nuclear DNA using validated multiplex kits, STR profiling generates a genotype that can individualise an animal within a reference population database.
Related terms
- Mitochondrial DNA (mtDNA)
- DNA located in mitochondria rather than the cell nucleus. Present in hundreds to thousands of copies per cell, making it recoverable from...
- Canine STR Multiplex
- A validated set of STR loci used to generate a genotypic profile from dog DNA. The International Society for Animal Genetics (ISAG)...
- Cementum
- A thin, bone-like mineralised tissue that covers the external surface of the tooth root. Deposited incrementally throughout life in alternating translucent and...
- Cryogenic Grinding
- The technique of freezing a tooth sample to cryogenic temperatures (liquid nitrogen, approximately -196°C) before mechanical pulverisation. Freezing prevents frictional heat generation...
- Dentinal Tubules
- Microscopic channels running radially through dentine from the pulp cavity to the outer surface. They house odontoblast cell processes that can carry...
- Medullary Index
- The ratio of the medulla diameter to the total hair shaft diameter. In companion animal hair identification, a medullary index above 0.5...
- Primary Transfer
- The direct movement of biological material from an animal to a surface it contacts. When a dog sheds hair onto its owner's...
- Pulp Extraction
- Accessing the soft tissue of the root canal (pulp) directly by cutting the tooth at the cementoenamel junction and removing the contents...
- Veterinary Forensic Pathology
- The application of post-mortem examination principles to animal deaths where criminal conduct is suspected. A veterinary forensic pathologist documents injuries, assesses cause...
Explained in these topics
- Companion Animal and Veterinary Forensic BiologyThe amplification and typing of microsatellite loci where short DNA sequences are repeated a variable number of times. When applied to canine or feline nuclear...
- Dental DNA, Tooth Sampling and Molecular IdentificationThe standard nuclear DNA typing method that examines the number of repeated sequence units at a set of polymorphic loci (typically 15-24 in modern commercial k...