RHD genotyping
Definition
PCR-based molecular typing of the RHD gene to determine D antigen status from DNA, including from dried biological evidence where serological red cell typing is not possible. Can also detect weak D, partial D, and DEL variants that serological methods may misclassify.
Related terms
- CDE nomenclature (Fisher-Race)
- The modern standard notation for Rh antigens, naming three antithetical pairs: C/c, D (no d), and E/e. Haplotypes such as CDe (R1),...
- D antigen
- The most immunogenic Rh antigen, encoded by the RHD gene. Its presence defines Rh-positive; its absence defines Rh-negative. No naturally occurring anti-D...
- Indirect antiglobulin test (IAT)
- A two-stage serological test in which IgG antibody is incubated with red cells, unbound antibody is washed away, and anti-human globulin (Coombs...
- Rh-Hr nomenclature (Wiener)
- An earlier notation system using alphanumeric agglutinogen codes (R0, R1, R2, Rz, r, r', r'', ry) for Rh haplotypes. Wiener's R1 corresponds...
- Weak D (formerly Du)
- A reduced-expression variant of the D antigen that does not agglutinate red cells with standard IgM anti-D reagent in the direct test...
Explained in
- The Rh Blood Group System in Forensic ContextPCR-based molecular typing of the RHD gene to determine D antigen status from DNA, including from dried biological evidence where serological red cell typing i...