Quantification Cycle
Definition
The cycle-associated threshold measurement used by an appropriate real-time PCR analysis. Input mass estimates the DNA quantity entering a reaction. Concentration, input mass and amplification behaviour are different quantities. A lower or higher cycle value is not a universal direct measure across assays without appropriate calibration.
- Context
- DNA measurement
For example, comparing cycle values from different assays without their calibration and thresholding conventions can produce a misleading quantity comparison.
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