Input Mass
Definition
Input mass is the estimated amount of DNA introduced into a downstream reaction, determined from the extract concentration and volume added under the measurement method. It differs from concentration and does not by itself establish target quality or amplification success.
- Context
- DNA measurement
For example, changing the volume added to a reaction changes estimated input mass even when the extract’s concentration stays the same.
Concentration, input mass and amplification behaviour are different quantities. A lower or higher cycle value is not a universal direct measure across assays without appropriate calibration.
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Explained in
- DNA Extraction and Quantification from Biological EvidenceThe amount of DNA estimated to enter a downstream reaction, distinct from concentration.