Mismatch
Definition
A non-standard pairing at a position, whose effect depends on context and assay conditions. A frameshift alters a coding reading frame through a length change not divisible by three. Consequences depend on sequence context and the assay or biological question.
- Context
- Sequence interpretation
For example, a non-standard base pairing can affect primer binding differently according to its position and the reaction conditions.
Neither term alone establishes a clinical effect or a forensic source association.
Related terms
- Amplicon
- The double-stranded DNA fragment produced between two PCR primers. In forensic STR typing, amplicon size determines whether a locus can be recovered...
- Denaturation
- Denaturation is disruption of a biological molecule’s usual structure under specified conditions. In PCR, it separates paired DNA strands sufficiently for subsequent...
- Duplex
- A nucleic-acid duplex consists of two associated complementary strands forming a paired structure. DNA strands in a typical duplex have opposite orientations;...
- Reverse Complement
- Complementary sequence written in the opposite strand’s usual 5-prime to 3-prime direction. Writing a reverse complement requires both complementary bases and reversal...