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Malachite Green

Definition

A synthetic triphenylmethane dye with an intense blue-green colour in its oxidised form. It is a common industrial dye and the coloured product of a positive LMG blood test.

Chemical class
Triphenylmethane dye
Oxidised colour
Intense blue-green
Common non-forensic use
Industrial and textile dye
Forensic use
Colour product of a positive LMG blood test

Common questions

How is malachite green related to the leucomalachite green blood test?+

Leucomalachite green is the colourless reduced form of malachite green; reaction with hemoglobin's peroxidase-like activity oxidises it back to malachite green, producing the visible blue-green colour that signals a presumptive positive.

Does a malachite green colour change confirm the presence of human blood?+

No. Like most presumptive blood tests, a positive colour change indicates possible blood but can be triggered by other oxidising substances, so a confirmatory test is needed to establish the stain is actually human blood.

Related terms

Cross-Checking
The practice of running two different presumptive tests on adjacent cuttings from the same stain. Concordant results (both positive or both negative)...
Leucomalachite Green (LMG)
The reduced, colourless form of malachite green. It is oxidised to the coloured malachite green form by active oxygen released when haemoglobin...
Presumptive Colour Test
Any rapid field or laboratory test that produces a colour change in the presence of a target substance. Positive results are indicative,...
Substrate Control
A sample of the unstained surface material from an area adjacent to a body fluid stain, collected alongside the stain and processed...
Triphenylmethane Dye
A class of synthetic dyes built around a central carbon bonded to three phenyl groups. Members include malachite green, crystal violet, and...

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