Human-Target Assay
Definition
An assay designed and assessed to examine specified human genetic targets. It may be used on material recovered from insects under a suitable validation. The presence of abundant insect DNA does not automatically prevent human-target amplification.
- Context
- Molecular analysis
For example, a human-specific target assay can examine a digestive-tract extract without requiring all nonhuman DNA to have been physically removed first.
Suitability, inhibition and contamination require the actual method and results.
Related terms
- Blood Meal
- Blood ingested by a feeding arthropod, potentially containing host material. Digestion, elapsed time and handling influence what is recoverable. A donor association...
- COI Barcoding
- Comparison of a defined mitochondrial COI region with suitable references for taxonomic identification. COI is widely used for animals, while appropriate markers...
- Gut Content Extraction
- Recovery of material from the digestive tract under a documented method with contamination controls. A positive result does not automatically establish feeding...
- Haematophagous Insect
- An insect that feeds on blood; its meal does not by itself establish the donor’s location or time of contact. Blood-feeding arthropods...
- Human-Contributor Mixture
- Genetic findings containing contributions from more than one human, distinct from merely containing nonhuman DNA. A human mixture involves more than one...
- Mitochondrial DNA (mtDNA) Haplotype
- A combination of linked mitochondrial variants that may be shared by maternal relatives and other individuals. A haplotype describes variants inherited together....
- Mitochondrial Haplotype
- A sequence pattern requiring lineage-aware comparison and relevant population interpretation. A haplotype describes variants inherited together. Mitochondrial haplotypes reflect maternal inheritance and...
- Mixed-Source Profile
- A profile involving multiple contributors or targets; insect and human DNA in one extract is not automatically a mixture of human contributors....