Gut Content Extraction
Definition
Physical dissection of a larva to remove the crop and midgut contents, which are then extracted separately from the larval carcass. The aim is to recover human DNA from the partially digested tissue in the gut without being overwhelmed by the vastly more abundant insect genomic DNA.
Related terms
- Blood Meal
- Blood ingested by a haematophagous insect during feeding on a vertebrate host. The host's DNA persists in the insect's gut for hours...
- COI Barcoding
- DNA-based species identification using a standard 658 bp segment of the mitochondrial cytochrome oxidase subunit I gene. Sequences are matched against reference...
- Haematophagous Insect
- An insect that feeds on the blood of a vertebrate host. Examples include mosquitoes (Culicidae), sand flies (Phlebotomus, Lutzomyia), and certain midges...
- Mitochondrial DNA (mtDNA) Haplotype
- A characteristic sequence of the mitochondrial genome used to assign an individual to a maternal lineage. More copies per cell than nuclear...
- Mixed-Source Profile
- A DNA profile resulting from a mixture of two or more contributors. Gut content extractions routinely produce mixtures of insect DNA and...
Explained in
- Insects as Alternate Samples for Toxicology and DNAPhysical dissection of a larva to remove the crop and midgut contents, which are then extracted separately from the larval carcass. The aim is to recover human...