ForenSeq DNA Signature Prep Kit
Definition
Verogen MPS library-preparation kit typing 27 autosomal STRs, 7 X-STRs, 23 Y-STRs, 94 identity SNPs, and 56 ancestry SNPs in a single workflow on the MiSeq FGx.
- Manufacturer
- Verogen
- Autosomal STRs
- 27
- X-STRs / Y-STRs
- 7 X-STRs, 23 Y-STRs
- SNP content
- 94 identity SNPs, 56 ancestry SNPs
- Instrument
- MiSeq FGx
Common questions
How does typing 27 autosomal STRs compare to the marker count used in conventional CODIS profiling?+
Standard CODIS core loci number 20 in the United States, so 27 autosomal STRs on this kit gives extra loci beyond the core set, increasing discriminatory power and improving compatibility across international STR databases that use slightly different core loci sets.
What investigative value do the 56 ancestry SNPs add in a case with no STR database match?+
When an unknown contributor's STR profile has no match in a criminal database, ancestry SNPs can estimate broad biogeographic origin, giving investigators a lead to narrow the pool of possible identities, something STR typing alone cannot provide.
Why include both X-STRs and Y-STRs alongside autosomal STRs in the same kit?+
X-STRs and Y-STRs are particularly useful in kinship and lineage testing, such as distinguishing close female relatives or tracing paternal lineage, complementing the autosomal STRs' general identity power for cases involving degraded mixtures or complex family relationships.
Related terms
- DOJ Interim Policy 2019
- US Department of Justice Interim Policy on Forensic Genetic Genealogy DNA Analysis (24 September 2019), limiting IGG to violent crimes and unidentified...
- GEDmatch PRO
- The law-enforcement tier of GEDmatch, acquired by Verogen in 2020. Operates on an opt-in model; only profiles of users who have explicitly...
- Identical by Descent (IBD)
- A chromosomal segment shared between two individuals because both inherited it from a common ancestor without an intervening recombination event. The amount...
- Investigative Genetic Genealogy (IGG)
- A forensic identification technique that uploads a crime-scene DNA profile to a consumer genealogy database, identifies partial matches suggesting distant family relationships,...
- Massively Parallel Sequencing (MPS) / NGS
- High-throughput sequencing technology that generates millions of short reads simultaneously; used in forensics for sequence-level STR typing (Verogen MiSeq FGx, Ion S5)...
- Othram Inc.
- Texas-based forensic genetics company offering the FORCE target-capture SNP profiling service for degraded and low-quantity crime-scene DNA and unidentified-remains casework using IGG.
- Parabon NanoLabs
- A US company that became the primary commercial provider of forensic IGG casework following the Golden State Killer case, offering the Snapshot...
- Precision ID GlobalFiler NGS STR Panel
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