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Crossed Nicols

The configuration in which the polariser (below the condenser) and the analyser (above the objective) are oriented at 90 degrees to each other, extinguishing all transmitted light unless the specimen has altered the polarisation state.

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Related terms

AutofluorescenceBirefringenceConoscopic illuminationDAPIDichroic mirrorEpi-fluorescence illuminationExtinction angleMichel-Lévy chartPhotobleachingRetardationStokes shift

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