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Becke Line

Definition

The bright optical halo at the edge of a solid fragment immersed in a liquid, visible under the microscope; moves toward the higher-RI medium when focus is raised. Its disappearance marks the RI match condition.

Appearance
Bright optical halo at a fragment's edge in immersion liquid
Behaviour on raising focus
Moves toward the higher refractive-index medium
Match indicator
Line disappears when solid and liquid RI are equal
Use
Glass and fibre refractive index determination

Common questions

Why raise the microscope focus rather than lower it to read the Becke line?+

Raising the objective moves the focal plane upward, which by convention causes the Becke line to migrate into the medium of higher refractive index, giving a directional cue that lowering focus would reverse, so the raising convention keeps readings consistent between analysts.

How does the Becke line method compare to GRIM for glass RI matching?+

The Becke line is a manual, visual immersion technique that finds the temperature or liquid at which the line vanishes. GRIM automates the same underlying principle by heating the immersion oil and using a video system to detect the match point instrumentally, giving more reproducible, quantitative results.

Related terms

ASTM E1967
The ASTM International standard for forensic glass RI measurement; specifies three acceptable methods (automated, semi-automated, manual), minimum precision of ±0.0001 RI units,...
Bayesian Likelihood Ratio
A statistical framework for expressing the evidential value of a comparison: the ratio of the probability of the observed RI similarity given...
Cauchy Equation
The empirical equation n(λ) = A + B/λ² + C/λ⁴ that describes the wavelength dependence (dispersion) of a material's refractive index; used...
Dispersion
The variation of refractive index with wavelength; all glasses and immersion oils show dispersion, which is why RI must be specified at...
ENFSI ENG3-2013
The ENFSI Glass Expert Working Group guideline for glass comparison casework; specifies the examination sequence, within-source variation requirements, statistical comparison framework, and...
GRIM-3
Glass Refractive Index Measurement, third-generation instrument by Foster + Freeman (UK); an automated phase-contrast hot-stage system that identifies the RI match point...
Immersion Method
The forensic technique of matching a glass fragment's RI to that of a reference oil by adjusting temperature; the fragment becomes invisible...
Locke 1985 Protocol
The calibrated immersion-oil series and operational procedure for forensic glass RI measurement described by Locke and Underhill (1985); specifies the oil series,...
Mettler FP-90
A general-purpose laboratory hot stage widely used in forensic glass RI measurement to provide ±0.1 °C temperature control for the manual Becke-line...
Phase-Contrast Microscopy
An optical technique that converts phase differences (RI differences at an interface) into intensity differences visible in the image; exploited by the...
Refractive Index (RI)
A physical property of glass measuring how much light bends as it passes through. RI is the primary discriminating characteristic used in...

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