Acid fraction

The organic phase recovered when the biological aqueous is acidified to pH 2 to 3 and shaken with diethyl ether or chloroform. Contains drugs with pKa below 7, mostly acidic compounds: barbiturates, salicylates, NSAIDs, paracetamol, glutethimide and phenols.

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Acidic, Neutral and Alkaline Drug Analysis

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Acid fraction

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