A-addition
The non-templated addition of a single adenine nucleotide to the 3' end of PCR products by Taq polymerase, exploited in forensic STR kits via a 60-minute final extension to drive all alleles to the +A form for consistent CE sizing.
The non-templated addition of a single adenine nucleotide to the 3' end of PCR products by Taq polymerase, exploited in forensic STR kits via a 60-minute final extension to drive all alleles to the +A form for consistent CE sizing.
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