Stas-Otto Procedure
Definition
Classical 1851/1856 acid-base liquid-liquid fractionation. Acidify with tartaric acid and extract acidic and neutral organics into ether, then alkalise and extract bases into chloroform. Yields four fractions used to separate barbiturates, phenols, alkaloids and volatiles.
Related terms
- ASE
- Accelerated solvent extraction (Dionex ASE). Pressurised solvent at elevated temperature pushes the analyte off the tissue matrix in minutes, replacing overnight Soxhlet.
- Conway Microdiffusion Cell
- A two-compartment glass or ceramic dish with a central well and an outer ring, sealed at the rim. The poison is released...
- QuEChERS
- Quick, Easy, Cheap, Effective, Rugged and Safe sample-prep protocol for pesticide residues. Acetonitrile extraction, MgSO4 + NaCl partition, dispersive SPE clean-up with...
- SLE
- Supported liquid extraction. Diatomaceous earth columns absorb the aqueous sample as a thin film and a single pass of organic solvent elutes...
- SPE
- Solid-phase extraction. Sample is loaded onto a packed sorbent cartridge (C18, mixed-mode MCX/MAX, polymeric Oasis HLB), washed and selectively eluted. The dominant...
- SPME
- Solid-phase microextraction. A polymer-coated fused-silica fibre (typically PDMS or PDMS-DVB) is exposed to vial headspace, then thermally desorbed in the GC injector....
- Steam Distillation
- Passing live steam through a sample so that volatile organic compounds co-distil with water at a temperature below their own boiling point....
- Viscera
- The standard set of organs and fluids collected at a suspected poisoning autopsy in India: stomach with contents, a length of upper...
Explained in
- Extraction, Isolation and Clean-up of Poisons from VisceraClassical 1851/1856 acid-base liquid-liquid fractionation. Acidify with tartaric acid and extract acidic and neutral organics into ether, then alkalise and ext...