Stas-Otto method

Sequential acid-alkaline solvent partitioning that isolates organic poisons from biological matrix in two fractions: acidic ether (Fraction A) for acidic and neutral drugs, and alkaline chloroform (Fraction B) for alkaloids. Developed by Jean Servais Stas in 1851 and modified by Julius Otto.

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Classical Extraction: Stas-Otto and Ammonium Sulphate Methods

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Stas-Otto method

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