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Rhodamine 6G

Definition

A fluorescent dye applied as a post-treatment to cyanoacrylate-developed fingerprints; excited at 530 nm (green), it emits at 565-590 nm (yellow-orange), greatly increasing contrast when examined under ALS through a matching barrier filter.

Dye type
Xanthene fluorescent dye
Excitation wavelength
488-530 nm (green light)
Emission color
Orange-red to yellow-orange
Application method
Dipping or spraying after cyanoacrylate fuming

Common questions

What is Rhodamine 6G used for in fingerprint analysis?+

Rhodamine 6G is a fluorescent dye applied after cyanoacrylate fuming to enhance the visibility of developed fingerprints on non-porous surfaces. It makes prints glow brightly under alternate light sources, dramatically improving contrast against the background.

How does Rhodamine 6G work under alternate light examination?+

The dye absorbs light at specific wavelengths (around 488-530 nm green light) and fluoresces bright orange-red or yellow-orange emission. Examiners use matching barrier filters to isolate this glow and capture high-contrast fingerprint images.

Is Rhodamine 6G the standard dye in fingerprint labs?+

Yes, it is the most widely used post-fuming fluorescent dye in UK, Australian, and North American forensic fingerprint laboratories. It works consistently on cyanoacrylate-developed prints and is typically applied by dipping or spraying after the fuming step.

Related terms

Alternate Light Source (ALS)
A device emitting light at selected wavelengths (typically 415 to 535 nm) used to detect biological fluids that fluoresce or absorb light...
Crime-Lite
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1:1 Macro Photography
Photography at a reproduction ratio of 1:1 (image sensor size equals subject size); the mandatory standard for all latent fingerprint photography before...
AFIS Workstation
A dedicated computer system with encoding software and database connectivity used by fingerprint examiners to encode latent minutiae, submit searches against criminal...
Ardrox
A commercial fluorescent dye mixture (originally for aircraft-skin crack detection) that fluoresces broadly under 365 nm UV illumination; used as a post-CA...
Bandpass Filter
An optical filter transmitting a defined wavelength range (typically 20-40 nm FWHM) while blocking all others; the excitation filter in an ALS...
Barrier Filter
An optical long-pass filter placed over the camera lens and worn as goggles by the examiner during ALS examination; blocks the excitation...
Basic Yellow 40 (BY40)
A fluorescent dye (excitation 450-490 nm, emission ~530 nm) applied immediately after CA fuming with a simple aqueous rinse to remove excess;...
Chain of Custody
The documented chronological record of who collected, handled, transferred, and examined a piece of evidence. For digital evidence, chain of custody includes...
Cyanoacrylate (CA) Fuming
The development of latent prints on non-porous surfaces by exposing the item to ethyl or methyl cyanoacrylate vapour in a humidity-controlled environment;...
Cyanoacrylate Fuming
A fingerprint development technique in which ethyl cyanoacrylate vapour polymerises onto eccrine sweat residue on a latent print, producing a stable, white...
DFO (1,8-Diazafluoren-9-One)
A fluorescent ninhydrin analogue used for latent fingerprint development on porous surfaces; excite at 450-470 nm (with secondary absorption extending into the...

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