Phosphoroscope
Definition
A rotating chopper that alternately blocks the excitation beam and the detector. Fluorescence dies during the blocked window, phosphorescence persists, so only phosphorescence reaches the PMT.
Related terms
- Fluorescence
- Light emission following absorption of light at a shorter (higher-energy) wavelength. Fluorescein absorbs blue-green excitation light and emits yellow-green light. The emitted...
- Jablonski Diagram
- An energy-level diagram showing the ground state, singlet excited states, and triplet state of a molecule, with arrows indicating absorption, internal conversion,...
- Phosphorescence
- Light emission from the triplet excited state (T1) via a spin-forbidden radiative transition; characterised by emission persisting after the excitation source is...
- Quantum Yield (Φ)
- Photons emitted divided by photons absorbed. A unit-less efficiency between 0 and 1. Quinine sulphate in 0.5 N sulphuric acid sits at...
- Stokes Shift
- The wavelength difference between the excitation peak and the emission peak of a fluorophore. A positive Stokes shift (emission at longer wavelength...
Explained in
- Fluorescence and Phosphorescence SpectroscopyA rotating chopper that alternately blocks the excitation beam and the detector. Fluorescence dies during the blocked window, phosphorescence persists, so only...