Ouchterlony Double Diffusion
Definition
A gel diffusion technique in which antigen and antibody placed in separate wells in an agar gel diffuse passively toward each other. A precipitation arc forms at the zone of equivalence. Also called immunodiffusion or the Ouchterlony technique after its developer, Orjan Ouchterlony.
Related terms
- Precipitin Reaction
- The formation of an insoluble antigen-antibody complex (precipitate) when soluble antigen and antibody meet at equivalent concentrations. The Ouchterlony double-diffusion test and...
- Anti-Human Serum
- Antibodies raised in a rabbit (or goat) by repeated injection of human serum. The rabbit's immune system makes IgG against human serum...
- Antigen-Antibody Reaction
- The specific non-covalent binding between an antigen (a molecule capable of eliciting an immune response) and the antibody produced against it. In...
- Antiserum
- Serum collected from an immunised animal containing antibodies directed against a specific antigen or group of antigens. In species identification, antiserum raised...
- COI Barcoding
- DNA-based species identification using a standard 658 bp segment of the mitochondrial cytochrome oxidase subunit I gene. Sequences are matched against reference...
- Counter-Immunoelectrophoresis (CIE)
- An accelerated form of gel diffusion in which an electric current drives antigen and antibody toward each other rather than relying on...
- Cross-Reactivity
- The capacity of an antibody raised against one analyte to bind structurally related compounds. In RIA, cross-reactivity is the main driver of...
- Cyt-B PCR
- Amplification and sequencing of the mitochondrial cytochrome-b gene. The reference marker for species identification in degraded and trace samples.
- Immunoelectrophoresis
- A two-step technique: proteins are first separated by electrophoresis in agar gel, then antiserum diffuses from a parallel trough and forms precipitin...
- Uhlenhuth Test (1901)
- The original precipitin ring test devised by Paul Uhlenhuth in Greifswald. Layering anti-human serum under a dilute bloodstain extract gives a white...
- Zone of Equivalence
- The concentration range at which antigen and antibody molecules are present in the correct ratio for maximum lattice formation and visible precipitation....
Explained in these topics
- Determination of Species of Origin from Blood and Body FluidsAntigen and antibody diffuse from separate wells in an agar gel; a precipitin line forms where they meet at equivalence. Useful for comparing several antisera...
- The Precipitin Test for Species IdentificationA gel diffusion technique in which antigen and antibody placed in separate wells in an agar gel diffuse passively toward each other. A precipitation arc forms...