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Numerical Aperture

Definition

n sin α for the relevant specimen-side medium and acceptance-cone half-angle. Numerical aperture depends on refractive index and the acceptance-cone half-angle. It contributes to collection and resolution properties under the actual imaging conditions.

Context
Microscope optics

For example, increasing image magnification does not replace the role of the optical system’s numerical aperture in the detail it can resolve.

Magnification alone does not describe resolved detail. Illumination, wavelength, specimen preparation and the rest of the optical system also matter.

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