Method validation parameters

The numbers a forensic method must hit before it is considered fit for routine use: linearity R² > 0.999 over the working range, LOD and LOQ at signal-to-noise of 3 and 10, intra-day and inter-day precision RSD below 2 to 5 percent, accuracy or recovery of 90 to 110 percent, and robustness against small mobile-phase or temperature changes. The Indian Pharmacopoeia and the FSSAI manuals codify these acceptance criteria.